The Incorporation of Glycine-1-C14 by Typhus Rickettsiae
نویسندگان
چکیده
منابع مشابه
The incorporation of glycine-1-C14 by typhus rickettsiae.
A previous study has shown that under suitable conditions typhus rickettsiae can incorporate small but significant quantities of labeled methionine. For maximal uptake, adenosine 5’-triphosphate, glutamine, all of the naturally occurring amino acids, the mononucleotides, diphosphopyridine nucleotide, glutathione, and a soluble protein fraction from normal yolk sac tissues were required (1). In ...
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Although it has been known for some time that rickettsiae have an independent, if limited, oxidative metabolism (Bovarnick and Snyder, 1949), evidence of any synthetic activity on the part of these organisms has until recently been lacking. It was noted (Bovarnick, 1956) that they were capable through oxidative phosphorylation of forming ATP,2 a compound that appears to be a prerequisite for al...
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Although it has been known for some time that rickettsiae have an independent, if limited, oxidative metabolism (Bovarnick and Snyder, 1949), evidence of any synthetic activity on the part of these organisms has until recently been lacking. It was noted (Bovarnick, 1956) that they were capable through oxidative phosphorylation of forming ATP,2 a compound that appears to be a prerequisite for al...
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Partially purified suspensions of typhus rickettsiae have been shown to exhibit metabolic activity as evidenced by consumption of oxygen and production of carbon dioxide in the presence of glutamate. Similar activity at a much lower rate occurs in the presence of pyruvate. The rate of oxygen uptake was directly proportional to the concentration of viable rickettsiae, as estimated by their toxic...
متن کاملThe incorporation of formate-C14, glycine-2-C14, adenine-4, 6-C14, and phosphate-P32 into nucleic acids.
nine labeled either with C'4 or N'5. Considerable controversy has developed as to which one of these tracer compounds gives an accurate measurement of the “relativeturnover-rate― of the nucleic acids. In order to determine the “absolute turnover rate― of the nucleic acids, it is necessary to know the specific activity of the immediate precursor of the nucleic acids. Since even the ident...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1960
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)76871-9